How to Buy Cell Culture Flask
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Posted On :
Mar-18-2015
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Article Word Count :
450
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This article has been written provide information about cell culture and its benefits. Also, it offers information about some basic problems encountered during the process. If you are the one who doesn’t have any idea about it, this article is for you.
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This article has been written provide information about cell culture and its benefits. Also, it offers information about some basic problems encountered during the process. If you are the one who doesn’t have any idea about it, this article is for you.
In fewer words, cell culture is the process of removing cells from a living organism and growing them in a controlled condition (a culture). This method is used to maintain cell lines. It is beneficial in culturing highly specialized cells under strictly defined conditions. The process allows quick access to growing cells to observe morphological changes. With the help of this process, a greater understanding of the workings of the cells can be accomplished without harming to a living being. Research in cells has provided many significant ways to new treatments for a range of serious health complications including spinal cord injury and burn, diabetes, and burns etc.
There are many common problems encountered during the process of cell culturing. And problems in primary cell culture may be present due to different reasons. Some of the most common problems are mentioned below:
Rapid ph shift in medium: There are many causes of this problem. Some of them are mentioned below:
* Incorrect Carbon dioxide tension
* Overly tight caps on tissue culture flask
* Insufficient bicarbonate buffering
* Incorrect salts in medium
* Bacterial, yeast, or fungal contamination
Precipitate in medium, no change in ph: Causes of it are mentioned below:
* Frozen medium
* Residual phosphate leftover from detergent washing, which may precipitate powdered medium components
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Cells are not adhered to the culture vessel: Some of the main reasons of it are explained below:
* Overly trypsinized cells
* Mycoplasma contamination
* No attachment factors in medium
Decreased growth of culture: The cause of this problem might be change in current cell culture medium, fetal bovine serum or other sera.
There are several apparatus and equipment generally considered essential for molecular biology laboratory. And without any doubt tissue processing for DNA identification is often an important part of molecular biology and many processes required for this. Some general apparatus include CO2 incubator for cell cultures, vacuum pumps, liquid nitrogen pumps, water bath, Chromatograph, Autoclaves and sterilizers, UV transilluminator, tubes, beakers, desiccator, pipettes with filters and flasks, necessary clamps, frames, stoppers, support, precision balances and many more. Either you can buy these apparatus from the market or you can opt for online shopping. There are many leading companies offering life science research products at very competitive rates. What you have to do is just visiting their websites and order. The products will be delivered at your doorsteps.
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Article Source :
http://www.articleseen.com/Article_How to Buy Cell Culture Flask_314455.aspx
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Author Resource :
The author is a freelance writer. He has written many articles and blogs on various topics related to life science research products. get more details: www.socalbiomedical.com
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Keywords :
cell culture flask, precision balances ,
Category :
Business
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Business
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