Biodetection Technologies - 6th Edition --- Aarkstore Enterprise

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<h1>Biodetection Technologies - 6th Edition --- Aarkstore Enterprise</h1><strong> Author: <a href='http://www.articleseen.com/profile_Aarkstore Enterprise.aspx'>Aarkstore Enterprise</a></strong><br /><p>The 6th edition in our Detection Technology series is an internationally recognized publication for experts in detection & identification of biological threats. This publication will explore the latest R&D developments including: • Point-of-care/clinical applications for pathogen/virus/threat detection and identification • Technological challenges for rapid/early/specific sensitive detection • Role of nanotechnology and system miniaturization • Bioinformatics for biodefense • Reducing false positives vs. detector sensitivity • Field-deployable devices: portability/compatibility/reliability/scalability • Reagentless vs. reagent biodetection systems • Non-PCR vs. PCR based detection techniques • Advances in microarray and sequencing technologies • Issues of sensitivity/testing/evaluation/validation Table of Contents : CHAPTER 1 Multiplex Real-Time PCR Assay Design for Pathogen Detection, Quantification and Speciation Robert S. Tebbs, PhD, Staff Scientist, Applied Biosystems* A multiplex real-time PCR assay was designed to identify 3 species of Vibrio and an internal positive control using a four-dye configuration. Multiple strains were sequenced to identify target sites. Several individual assays within the multiplex contain more than one primer or probe due to strain to strain polymorphisms. Vibrio cholerae, V. parahaemolyticus, and V. vulnificus were detected either individually or in mixtures at ≤25 genomic copies. The Vibrio multiplex assay showed 100% specificity to all targets analyzed and no detection of an exclusion panel that included nearest neighbors. Each assay in the multiplex exhibited 100 ± 10% efficiency over a 5 log range. Multiplex real-time PCR can simplify pathogen detection and reduce costs since 3 species can be analyzed in a single reaction tube. *In collaboration with: P.M.Brzoska, S.Bit, M.R.Furtado, and O.V.Petrauskene CHAPTER 2 Multiplexed Diagnostic Assays for Viral Pathogens of Cattle and Swine Max V. Rasmussen, PhD, Biomedical Scientist, Biosciences Defense Division, Lawrence Livermore National Laboratory Diagnostic tests for animal pathogens support a healthy livestock industry and reliable food supply. Lawrence Livermore National Laboratory developed multiplexed assays that simultaneously discriminate nucleic acid sequences of foreign and endemic viral pathogens affecting cattle and swine in a high-throughput format. The porcine-disease assay detects foot-and-mouth disease, vesicular stomatitis, swine vesicular disease, vesicular exanthema of swine and porcine reproductive and respiratory syndrome. The bovine-disease assay detects foot-and-mouth disease, malignant catarrhal fever, rinderpest, bovine viral diarrhea, bluetongue, bovine papular stomatitis, psuedocowpox, infectious bovine rhinotracheitis, vesicular stomatitis and bovine herpes mammalitis. Ongoing DHS-funded research includes enhancing sensitivity to FMDV, supporting the USDA-APHIS assay validation process and development of a FMDV sub-typing microarray. CHAPTER 3 Challenges for Sample Preparation and Pathogen Detection: Perspectives from the Food Industry Daniel R. DeMarco, PhD, Senior Research Microbiologist, DuPont Qualicon Many promising new sensor technologies purport to have potential applications in foodborne pathogen detection. Often there is little real understanding of the complexities and issues involved. In contrast to air or clinical samples which are relatively simple, food matrix presents a host of unique challenges that must be overcome before adoption of any particular sensing technology will occur in the food industry. These include but are not limited to; sensitivity requirements, sample size issues, matrix diversity, and presence of assay specific inhibitors. In addition, the food industry is notoriously low cost and often low tech in its historically preferred approaches to pathogen detection. This talk will highlight specific examples of the challenges faced for pathogen detection in foods, discuss some history and background of past and currently in-use technologies, and discuss future trends. For more information, please visit : http://www.aarkstore.com/reports/Biodetection-Technologies-6th-Edition-37211.html </p><strong>About the Author:</strong><br />Aarkstore Enterprise specialize in providing online market business information on market research reports, books, magazines, conference booking at competitive prices, and strive to provide excellent and innovative service to our customers.<p> Article Source: <a href='http://www.articleseen.com/Article_Biodetection-Technologies-6th-Edition-Aarkstore-Enterprise_39104.aspx'> http://www.articleseen.com/Article_Biodetection Technologies - 6th Edition --- Aarkstore Enterprise_39104.aspx</a></p>

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Keywords : Biodetection, Technologies, Edition, Detection, Technology, internationally, recognized, publication, detection, identificati,

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